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. 2020 Feb 3;9(2):e1107. doi: 10.1002/cti2.1107

Figure 2.

Figure 2

Memory B‐cell responses and long‐term antibody responses are increased in A‐eIIV‐vaccinated mice. BALB/c mice were vaccinated twice i.m. with either PBS, S‐IIV, A‐eIIV, H‐eIIV or R‐eIIV vaccines 3 weeks apart. iLN was collected at days 7 and 21 post‐vaccination to quantify by FACS (a), IgG+ (b) and IgM+ (c) B memory cells. Longitudinal serum samples were serially sampled from individual mice at 2, 4 and 6 months post‐vaccination for H3N2‐2013 (d), H3N2‐1968 (e) and H3‐stem (f) IgG by ELISA for avidity. Data represent the mean, SEM and individual responses, n = 4–8 mice per group. For (b, c), *shows statistical significance for eIIV versus S‐IIV by one‐way ANOVA (mixed model) and Tukey's multiple comparison test. For d–f, #shows statistical significance for low‐avidity antibodies and *for high‐avidity antibodies for eIIV versus S‐IIV by one‐way ANOVA (mixed model) and Tukey's multiple comparison test. # or *P < 0.05, ## or **P < 0.01, ### or ***P < 0.005, and #### or ****P < 0.001. Experiments were repeated twice. Representative FACS plots were gated on lymphocytes for FSC/SSC and live/dead (a).