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. 2020 Jan 30;13:295–310. doi: 10.2147/IDR.S226215

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© 2020 Ballesteros-Monrreal et al.

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Multiplex-PCR Banding Patterns of Virulence Genes and Individual PCRcnf of E. coli generated by gel electrophoresis. (A) iucD (512 bp), satA (384 bp), fliC (304 bp) and fimH (210 bp). (B) papGII (562 bp), papGIII (421 bp); vatA (330 bp), vatP (226 bp) and iha (150bp). (C) hlyA (1, 280 bp), satP (880 bp) and papA (641 bp). (D) cnf-1 (3, 100 bp). M. Molecular weight market (100 bp Plus DNA Ladder in A, B and C and 1 kb Plus DNA ladder in D) . Lane C+. Positive control (E. coli CFTO73 in A; E. coli CFTO73 plus E. coli O59I in B and E. coli GAG1 in C and D) . Lines with a number E. coli isolated from the samples. C-. Negative control.