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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Trends Mol Med. 2019 Nov 7;26(2):150–169. doi: 10.1016/j.molmed.2019.10.001

Table 1:

Clinical trials conducted to evaluate safety and efficacy of therapies to improve cognitive deficits in Down syndrome

Treatment Trial Id# Phase Participants Ages Use/MOA Pre–clinical Data Results References
Rivastigmine i
II
N/A
1, 2
14
42
8+, 10–17 yrs. AD/ Cholinesterase inhibitor Derivative or Physostigmine/Galantamine–Ts65Dn improve 4–arm maze, olfactory test No significant improvement in aspects of cognition, language, or overall function [101-103]
Donepezil/ E2020 III
IV
V
VI
VII
2
3
3
2
2
128
8
9
36
150
6–10, 11–17, 15–39 yrs. AD/ Cholinesterase inhibitor Ts65Dn– no change in MWM, Altered cholinergic system in DS Terminated due to lack of efficacy [104]
Memantine VIII
IX
X
4
N/A
2
42
180
200
18–32, 15–32, 40+ yrs. AD/ NMDA uncompetitive antagonist Evidence of cognitive improvement/ partial improvement in Ts65Dn–Contextual fear conditioning, MWM, WRAM, NOR No improvement in primary measures, p<0.10 improved CVLT–II scores, PAL Stages, DAS–II Recall of Digits [22, 105]
ELND005/scyllo–inositol XI 2 23 18–45 yrs. AD/Prevent/reduce amyloid aggregation, increase amyloid–β clearance and myo–inositol regulation to improve cognitive function Amyloid anti–aggregation effects in vitro, protective effects on oligomer–induced neuronal toxicity, and positive effects on learning in animal models of AD No differences in cognitive or behavioral measures, and there were no SAEs or deaths in the study, high dose groups discontinued [23]
ACI–24 XII 1 24 25–46 yrs. AD/ liposomal vaccine– induce anti–Aβ antibodies Ts65Dn non–significant reduction in brain Aβ levels, improved NOR and CFC, reduction of cholinergic neuron atrophy Ongoing [24]
Nicotine–transdermal XIII 1, 2 15 25+ yrs. Nicotinic Cholinergic No studies performed Ongoing
Basmisanil/ RO5186582/ RG1662 XIV
XV
XVI
XVII
2
1
1
2
173
35
13
45
6–11, 12–30, 18–30, 18–40 yrs. Inverse agonist/negative allosteric modulator of GABAAR Antagonism of GABA in Ts65Dn–improvement in NOR, MWM, Y–maze Terminated due to lack of efficacy. [29, 104]
BTD–001/pentylenetetrazole/PTZ ACTRN12612000652875XXIV 1 88 13–35 yrs. Non–competitive GABAAR antagonist Chronic treatment improved NOR, MWM, persisted after cessation of treatment Ongoing [21, 104]
Bumetanide 2015–005780–16XXV 2 24 10–16 yrs. Autism & Seizure trials/ Sulfamyl loop diuretic for heart failure/ blocks the NKCC1 cation–chloride co–transporter, decreases neuronal intracellular Cl– Rescue of LTP, NOR, OL in Ts65Dn Ongoing [32, 106]
EGCG XX
XXI
2
2
31
87
14–29, 14–39 yrs. DYRK1A inhibitor, lipid lowering properties, antioxidant Inhibition by EGCG– showed improved MWM, NOR, Y–maze, Some improvement in cognitive performance with EGCG and training [10, 107]
Folic Acid/ Folic Acid + Thyroid hormone XXII
XXIII
2, 3
3
120
175
3–30 mos., 6–18 mos. Vitamin– does not require dihydrofolate reductase for its conversion Genes related to folate metabolism are located on chromosome 21–leading to abnormal metabolism & low folate levels Some low–level positive impact on developmental age [13]
Vitamin E XXIV
XXV
3
3
350
349
50+ yrs. α–tocopherol, a lipophilic chain–breaking antioxidant, acts as an inhibitor of lipid peroxidation Ts65Dn improvement in EP–maze, MWM, R–maze Did not slow cognitive deterioration in older individuals with DS [108]

AD: Alzheimer disease, GABAAR-GABA-A receptor, NKCC1-Na-K-Cl-Cotransporter, MWM: Morris water maze, WRAM: water radial arm maze, NOR: novel object recognition, CFC: conditioned fear conditioning. LTP: long-term potentiation of synaptic activity, CVLT-II: California Verbal Learning Test 2nd ed., PAL: Paired Associates Learning task, DAS-II: Differential Ability Scales-II, SAE: Significant Adverse Effect