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. 2019 Dec 12;9(3):1141–1151. doi: 10.1002/cam4.2723

Figure 1.

Figure 1

Aloin, MET, and combination inhibited cell proliferation in vitro. A, HepG2 cells were treated with PBS, MET (400 μmol/L), aloin (50 μmol/L), and combination treatment (MET 400 μmol/L and aloin 50 μmol/L), the CCK‐8 assay was used to determine cell viability. B, Bel‐7402 cells were treated with PBS, MET (400 μmol/L), aloin (50 μmol/L), and combination treatment (MET 400 μmol/L and aloin 50 μmol/L), the CCK‐8 assay was used to determine cell viability. C, Effects on the colony formation in HepG2 and Bel‐7402 cells were determined for 24 h. D, Relative protein expression of Ki67 and PCNA was detected by western blotting in HepG2 cells. The representative column diagrams showing results of relative protein expression. E, Relative protein expression of Ki67 and PCNA was detected by western blotting in Bel‐7402 cells. The representative column diagrams showing results of relative protein expression. Date are represented as the mean ± SD of three independent experiments. *P < .05,**P < .01, compared with Control,#P < .05, compared with MET