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. 2020 Feb 3;10:1727. doi: 10.1038/s41598-020-58734-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of wt and sixteen LDLR variants in CHO-ldlA7 transfected cells. Expression of LDLR was determined 48 h post-transfection with the plasmids carrying the different LDLR variants by Western blot and flow cytometry. (A,B,E,F) Western blot analysis of LDLR expression. (C,G) Quantification of the intensities of the bands obtained by Western blot by densitometry and, (D,H) LDLR expression determined by flow cytometry. A representative blot is shown in panel A,B,E and F. C and G represent the mean of band quantification of three independent Western blots. The values in D and H represent the mean of triplicate determinations (n = 3); error bars represent ± SD. *P < 0.001 compared to wt using a Student’s t-test.