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. 2020 Feb 3;10:1659. doi: 10.1038/s41598-020-57671-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE of recombinant jrPPO1-wt at different purification stages under reducing conditions. M = marker, lane 1: lysate (70 µg), lane 2: soluble lysate (40 µg), lane 3: eluate of the 1^st GSTrap column (10 µg), lane 4: GST-jrPPO1-wt mixed with HRV-3C-protease for 40 hours and lane 5: pure latent jrPPO1-wt after the 2^nd GSTrap column. The red arrow indicates the over-expression band of the fusion protein (GST-jrPPO1-wt). The black arrow shows jrPPO1-wt after removal of the GST-tag. The gel was cropped to display the lanes of interest (full-length gel: Fig. S23).