Table 1.
Yields of heterologously expressed jrPPO1-wt and the five investigated mutants per liter of culture, copper content per enzyme and the molecular weight measured by ESI-MS.
| Yield (mg/l) | Cu-ions/enzyme | Mass (calculated) (Da) | Mass (measured) (Da) | Δ/Da | |
|---|---|---|---|---|---|
| jrPPO1-wt | 38 | 0.8 ± 0.1 | 56359.4 (−4 H) | 56359.5 | +0.1 |
| Phe260Gly | 39 | 1.0 ± 0.1 | 56269.3 (−4 H) | 56269.2 | −0.1 |
| Asn240Lys | 39 | 0.8 ± 0.1 | 56373.4 (−4 H) | 56373.4 | ±0 |
| Leu244Arg | 22 | 1.1 ± 0.1 | 56400.4 (−6 H) | 56400.4 | ±0 |
| Asn240Lys/Leu244Arg | 11 | 1.1 ± 0.1 | 56414.5 (−6 H) | 56414.6 | +0.1 |
| Asn240Thr/Leu244Arg | 12 | 0.9 ± 0.1 | 56387.4 (−6 H) | 56387.4 | ±0 |