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. 2019 Dec 31;11:10969–10982. doi: 10.2147/CMAR.S234327

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© 2019 Yu et al.

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ILCregs from normal donors and AML patients.

Notes: The different expression patterns of ILCregs from normal donors (right panel) and AML patients (left panel) are shown in Figure 1. Single-cell populations with SSC vs FSC were selected and were further gated on the CD45+Lin- population. Furthermore, gating of CD127+IL-10+ and CD127+IL-10- sub-population was done by different fluorescence-conjugated antibodies' combinations. Regulatory innate lymphoid cells were identified as CD45+Lin-CD127+IL-10+ population. At least a total of 1 million cells were collected for analysis of ILCreg cells.