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. 2020 Jan 20;12(2):292–305. doi: 10.4168/aair.2020.12.2.292

Table 1. Flow cytometry panels for primary immunodeficiency disease diagnosis.

Panels Target proteins (clone) Manufacturers
BTK panel BTK (53), CD14 (M0P9) Becton Dickinson
Double negative T-cell panel, ALPS CD3 (SK7), CD4 (SK3), CD8 (SK1), TCR-ab (WT31), TCR-gd (011F2) Becton Dickinson
Extended B cell panel CD10 (ALB1), CD19 (SJ25C1), CD24 (SN3), CD38 (T16), CD27 (0323), IgD (IA6-2) Beckman coulter, eBioscience, Becton Dickinson
Extended T cell panel CD45RA (HI100), CD45RO (UCHL1), CCR7 (150503) eBioscience, Becton Dickinson
Hyper-IgM panel CD40 (5C3), CD40L (TRAP1) Becton Dickinson
Leukocyte adhesion deficiency panel CD11b (ICRF44), CD18 (6.7) Becton Dickinson
Lymphocyte subset CD3 (SK7), CD19 (SJ25C1), CD56 (NCAM16.2), CD4 (SK3), CD8 (SK1) Becton Dickinson
Neutrophil respiratory burst test (DHR) NADPH oxidase Sigma-Aldrich
Perforin, HLH panel CD8 (SK1), CD56 (NCAM16.2), Perforin (8G9) Becton Dickinson
Regulatory T cell panel FOXP3 (PCH101), CD25 (2A3), CD4 (RPA-T4) eBioscience, Becton Dickinson
STAT1 phosphorylation panel CD3 (SK7), pSTAT1 (pY701) Becton Dickinson

BTK, Bruton's tyrosine kinase; ALPS, autoimmune lymphoproliferative syndrome; DHR, dihydrorhodamine; HLH, hemophagocytic lymphohistiocytosis; CD, cluster of differentiation; TCR, T-cell receptor; STAT1, signal transducer and activator of transcription 1; pSTAT1, phosphorylated signal transducer and activator of transcription 1; NADPH, nicotinamide adenine dinucleotide phosphate; CCR7, C-C chemokine receptor type 7; IgD, immunoglobulin D.