Table 1. Flow cytometry panels for primary immunodeficiency disease diagnosis.
Panels | Target proteins (clone) | Manufacturers |
---|---|---|
BTK panel | BTK (53), CD14 (M0P9) | Becton Dickinson |
Double negative T-cell panel, ALPS | CD3 (SK7), CD4 (SK3), CD8 (SK1), TCR-ab (WT31), TCR-gd (011F2) | Becton Dickinson |
Extended B cell panel | CD10 (ALB1), CD19 (SJ25C1), CD24 (SN3), CD38 (T16), CD27 (0323), IgD (IA6-2) | Beckman coulter, eBioscience, Becton Dickinson |
Extended T cell panel | CD45RA (HI100), CD45RO (UCHL1), CCR7 (150503) | eBioscience, Becton Dickinson |
Hyper-IgM panel | CD40 (5C3), CD40L (TRAP1) | Becton Dickinson |
Leukocyte adhesion deficiency panel | CD11b (ICRF44), CD18 (6.7) | Becton Dickinson |
Lymphocyte subset | CD3 (SK7), CD19 (SJ25C1), CD56 (NCAM16.2), CD4 (SK3), CD8 (SK1) | Becton Dickinson |
Neutrophil respiratory burst test (DHR) | NADPH oxidase | Sigma-Aldrich |
Perforin, HLH panel | CD8 (SK1), CD56 (NCAM16.2), Perforin (8G9) | Becton Dickinson |
Regulatory T cell panel | FOXP3 (PCH101), CD25 (2A3), CD4 (RPA-T4) | eBioscience, Becton Dickinson |
STAT1 phosphorylation panel | CD3 (SK7), pSTAT1 (pY701) | Becton Dickinson |
BTK, Bruton's tyrosine kinase; ALPS, autoimmune lymphoproliferative syndrome; DHR, dihydrorhodamine; HLH, hemophagocytic lymphohistiocytosis; CD, cluster of differentiation; TCR, T-cell receptor; STAT1, signal transducer and activator of transcription 1; pSTAT1, phosphorylated signal transducer and activator of transcription 1; NADPH, nicotinamide adenine dinucleotide phosphate; CCR7, C-C chemokine receptor type 7; IgD, immunoglobulin D.