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. 2020 Jan 28;9:1487. doi: 10.3389/fonc.2019.01487

Figure 3.

Figure 3

SSA suppresses the migration and invasion of TNBC cells and the lung metastasis of 4T1-luc cells. (A,B) Photographs and quantification of wounds to SUM149 and MDA-MB/231 cells treated with SSA. (C,D) Photographs and quantification of SUM149 and MDA-MB/231 cell migration through Transwell migration chambers with 8-μm pores and stained with 0.2% crystal violet. (E,F) Photographs and quantification of SUM149 and MDA-MB/231 cell invasion through Matrigel-coated polycarbonate membranes, with cells stained by 0.2% crystal violet. The independent experiments were performed in triplicate. *p < 0.05; ***p < 0.001 compared with 0 μM of SSA. (G) Luciferase imaging of lung metastases at 2 weeks after injection of 4T1-luc cells and treatment with paclitaxel, AMD3100, or SSA. (H) ROI analysis of images obtained at 2 weeks was used to quantify changes in cell numbers in lungs after treatment with paclitaxel, AMD3100, or SSA. The data represent mean values ± SEM for photon flux in each group (n = 5). **p <0.01, ***p <0.001 vs. control. (I) H&E staining of lung tissue of 4T1-luc mice. The photographs in the right row are magnifications of the boxes in the left row. Asterisks indicate bronchioles in lung tissue.