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. 2020 Jan 28;9:1487. doi: 10.3389/fonc.2019.01487

Figure 4.

Figure 4

SSA down-regulates CXCR4 to inhibit the migration and invasion of TNBC cells. (A) SSA inactivates the CXCR4 promoter in a concentration-dependent manner. SUM149 (B) and MDA-MB-231 (C) cells were incubated with SSA at various concentrations for 24 h. CXCR4 expression of cells was analyzed by Western blot. The histograms on the right of Western blots show the expression levels of CXCR4 in SUM149 and MDA-MB-231 cells. Data from three independent experiments were pooled. ***p < 0.001 vs. 0 μM of SSA. CXCR7 protein expression of SUM149 (D) and MDA-MB-231 (E) after incubating with SSA at various concentrations for 24 h. The histograms on the right of Western blots show the expression levels of CXCR7 in SUM149 and MDA-MB-231 cells. Data from three independent experiments were pooled. (F) Sections of xenografts (upper row) and lung tissue (lower row) in different groups were submitted to immunohistochemistry using antibodies of CXCR4. (G) Western blot analysis detection of the CXCR4 and CXCR7 protein expression in the lung tissue of 4T1-luc mice in different groups. (H) Western blot analysis of CXCR4 protein expression in SUM149 and MDA-MB-231 cells transfected with an siRNA against CXCR4 for 24 h. SUM149 and MDA-MB/231 cells were transfected with an siRNA against CXCR4 followed by treatment with SSA for 24 h at the indicated concentrations. Transwell (I,J) and invasion (K,L) assays were performed. The independent experiments were performed in triplicate. *p < 0.05; **p < 0.01; ***p < 0.001 compared with cells transfected with control siRNA.