Table 1.
Genome editing in stable transgenic rice lines with intron based Cas9 system
| Targeted rice sites | CRISPR scaffold | Tested T0 lines | Mutated T0 lines (number; ratio) |
Biallelic mutation lines (number; ratio) |
Constructs |
|---|---|---|---|---|---|
| OsDEP1-sgRNA01 | Cas9 (inO::tRNA) | 10 | 9, 90.0% | 7, 70.0% | pZHZ159 |
| OsDEP1-sgRNA02 | 12 | 5, 46.5% | 2, 16.7% | pZHZ160 | |
| OsPDS-sgRNA01 | 15 | 9, 60.0% | 2, 13.3% | pZHZ161 | |
| OsPDS-sgRNA02 | 26 | 14, 53.8% | 6, 23.1% | pZHZ162 | |
| OsDEP1-sgRNA01 | Cas9 (inO::RZ) | 14 | 10, 71.4% | 4, 28.6% | pZHZ167 |
| OsDEP1-sgRNA02 | 6 | 2, 33.3% | 1, 16,7% | pZHZ168 | |
| OsPDS-sgRNA01 | 18 | 11, 84.6% | 3, 16,7% | pZHZ169 | |
| OsPDS-sgRNA02 | 10 | 1, 10.0% | 1, 10.0% | pZHZ170 | |
| OsDEP1-sgRNA01 | Cas9 (inO::NU) | 25 | 11, 44.0% | 8, 32.0% | pZHZ171 |
| OsDEP1-sgRNA02 | 12 | 4, 33.3% | 0, 0.0% | pZHZ172 | |
| OsPDS-sgRNA01 | 11 | 3, 27.3% | 2, 16.7% | pZHZ173 | |
| OsPDS-sgRNA02 | 37 | 11, 29.7% | 1, 9.1% | pZHZ174 | |
| OsDEP1-sgRNA01 | Cas9 (inO::tRNA) | 30 | 17, 58.6% | 7, 23.3% |
pZHZ189 (Multiplex) |
| OsDEP1-sgRNA02 | 30 | 3, 10.0% | 0, 0.0% | ||
| OsDEP1-sgRNA01 | Cas9 (inO::tRNA) | 50 | 30, 71.4% | 20, 40.0% |
pZHZ190 (Multiplex) |
| OsPDS-sgRNA02 | 50 | 2, 4.0% | 0, 0.0% |