Table 2.
Genome editing in stable transgenic rice lines with intron based Cas12a system
| Targeted rice sites | CRISPR scaffold | Tested T0 lines | Mutated T0 lines (number; ratio) |
Biallelic mutation lines (number; ratio) |
Constructs |
|---|---|---|---|---|---|
| OsDEP1-crRNA01 | Cas12a (inO::DR-DR) | 15 | 7, 46,7% | 0, 0.0% | pZHZ144 |
| OsROC5-crRNA-01 | 22 | 2, 9.1% | 0, 0.0% | pZHZ146 | |
| OsDEP1-crRNA01 | Cas12a (inO:: HH-HDV) | 19 | 1, 5.3% | 0, 0.0% | pZHZ148 |
| OsROC5-crRNA-01 | 21 | 0, 0.0% | 0, 0.0% | pZHZ150 | |
| OsROC5-crRNA01 | Cas12a (inO:: HH-HDV) | 22 | 1, 4.5% | 0, 0.0% |
pZHZ208 (Multiplex) |
| OsROC5-crRNA02 | 22 | 3, 13.7% | 0, 0.0% |