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. 2020 Feb 3;8:4. doi: 10.1038/s41413-019-0081-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Wnt7b overexpression does not affect the cartilage callus of fractures. a A schematic of experimental design. b Representative X-ray images of femurs immediately following fracture (D0) and at 7 days after procedure (D7). c Representative H&E images of medial sections through the fractured femur at D7. P stabilizing pin, asterisk indicates cartilage callus. Note excessive bone accrual in distal metaphyseal region (#) of the +Dox mouse. d Measurements of cartilage callus areas on sections. N = 3 mice. Three sections per mouse were measured with the NanoZoomer NDP software.