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. 2020 Feb 3;11(2):80. doi: 10.1038/s41419-020-2283-9

Fig. 1. Study design.

Fig. 1

Mcl1∆hep mice and control littermates (Mcl1flox/flox, referred to as WT) were placed on standard chow or diet high in fat, fructose and cholesterol (FFC diet). a One cohort of mice was sacrificed after 4 months of feeding to assess NASH phenotype. Another cohort of mice was sacrificed after 10 months of feeding to assess tumor development. b Immunoblot analysis of Mcl1 in whole liver tissue of mice fed for 4 months. GAPDH serves as a loading control. c Densitometry of the Mcl1 immunoblot in the liver tissue. Mcl1 protein levels were normalized to GAPDH levels. Chow-WT n = 6 mice; Chow-Mcl1∆hep n = 5 mice; FFC-WT n = 6 mice; FFC-Mcl1∆hep n = 6 mice. d mRNA expression of anti-apoptotic proteins in whole liver tissue of mice fed for 4 months. Chow-WT n = 8 mice; Chow-Mcl1∆hep n = 5 mice; FFC-WT n = 12 mice; FFC-Mcl1∆hep n = 7 mice; Bars represent mean ± SEM. ***p < 0.001, **p < 0.01, *p < 0.05 or not significant (ns).