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. 2020 Feb 3;10:1656. doi: 10.1038/s41598-020-58222-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H&E and azan staining of orthotopic periodontal tissue injury site. (A–D) Natural periodontal tissue without any periodontal tissue injury, (E–H) Periodontal tissue injury, (I–L) Control, without any cell sheet transplantation after 8 weeks (black arrow heads show the epithelial tissue migration to the root surface) (M–P) PDL cell sheet transplant after 8 weeks, (Q–T) Complex cell sheet transplant after 8 weeks (PDL-like and bone-like tissue is demarcated using black dotted lines in H&E and yellow dotted lines in azan staining, black arrow points show the PDL fibers attached similar as in natural periodontal tissue to the root respectively).