TABLE 4.

Primers of deletion and complement the gene of cusR.

Name	Primer	5′→3′
△cusR	C3-△cusR-A	GTATGCGGCCGCCACGACGGGC TTCTTC
	C3-△cusR-B	AGGTCGTCTGGACGGTTTGCATG CTCCCCGGCTGGCTGC
	C3-△cusR-C	GCAGCCAGCCGGGGAGCATGCA AACCGTCCAGACGACCT
	C3-△cusR-D	GTATGCGGCCGCGATTCTCGGAG GTGATGTT
C-△cusR	C3-△cusR-F	GAGTCCATGGATGAAAATATTA ATCGTTGA
	C3-△cusR-R	GAGTGTCGACTGGAGATCCCGG ATGCATAG
pKO3-Km	pKO3-Km-F	AATAAGCGGATGAATGGCAG
	pKO3-Km-R	TCCCTCACTTTCTGGCTGG
pGEM-T-easy	pGEM-T-easy-km-F	GCGAATTGGGCCCGACGTC
	pGEM-T-easy-km-R	CGCAGCCGAACGACCGAG
cusR	C3-cusR-F	GGCACGATTGAACACAGGG
	C3-cusR-R	CCGAGAGGTCAGCCACTTT
16s rRNA	16s rRNA-F	AAAGCGTGGGGAGCAAACAG
	16s rRNA-R	CCGCTGGCAACAAAGGATAA

“__” enzyme cutting site; Nco I :CCATGG; Sal I :GTCGAC; NotI :GCGGCCGC.