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. 2020 Feb 3;10:1681. doi: 10.1038/s41598-020-58700-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of HCEnCs stored in 4 °C Endo-SFM(+) suspension. (A,B) HCEnCs were collected and analyzed with flow cytometry for expression of cell surface Prdx6 (sPrdx6) and CD166 before storage, immediately after storage, and after 2 days stabilization at 37 °C. Staining with an IgG mouse isotype antibody served as a negative control. Positive expression gates were set to include < 2% of isotype controls. (*p < 0.05, **p < 0.01; n = 4) (C) HCEnCs were re-seeded pre-storage, post-storage, and post-stabilization and processed with a Click-iT EdU kit to assess proliferative capacity (n = 3).