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. 2020 Feb 3;10:1692. doi: 10.1038/s41598-020-57884-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of RNA-Seq data using qPCR. Strong positive correlation of 18 differentially regulated genes (expression ratios ≥2 and Padj <0.05, n = 3) between RNA-Seq and qPCR data at (a) 6 h (R² = 0.90) and (b) 48 h (R² = 0.90) after hypoxia. Fold-changes represent the expression changes of each gene under hypoxia (6- or 48 h) relative to its respective control, (n = 3).