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. 2020 Jan 28;8:11. doi: 10.3389/fchem.2020.00011

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Copyright © 2020 Karppi, Zhao, Chong, Koistinen, Tenkanen and Master.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HILIC-MS/MS spectra collected in negative ion mode showing fragmentation of X₂ after enzymatic oxidation by PDHs followed by NaBD₄ reduction. (A) Negative MS/MS 320 of the X₂ control, (B) negative MS/MS 322 of the X₂ oxidized by AbPDH1, (C) negative MS/MS 322 of the X₂ oxidized by AmPDH1, (D) negative MS/MS 298 of the X₂ oxidized by AbPDH1, (E) negative MS/MS 299 of the X₂ oxidized by AmPDH1. Product ions named according to Domon and Costello (1988). Oxidations at C-2, C-3, and C-4 are not distinguishable.