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. 2020 Jan 28;10:1617. doi: 10.3389/fphar.2019.01617

Figure 4.

Figure 4

Rhynchophylline enhanced autophagy and attenuated Ang II-induced EPCs senescence. (A) The chemical structure of Rhy. (B) Western blot analysis of LC3-II, beclin-1 and p62 expression in EPCs in indicated groups. The data was presented in fold of control. (C) Representative images and quantitative analysis of mRFP-GFP-LC3 immunofluorescence (400× magnification). (D) Autophagosomes were observed by transmission electron microscopy. (E) Representative images and quantitative analysis of SA-β-gal activity assay (100× magnification). (F) Telomerase activity was determined by telomeric repeat amplification protocol assay and indicated by the difference value (OD = A450-A690). (G) Western blot analysis of p53 and p21 expression in EPCs in indicated groups. The data was presented in fold of control. All data were pooled as mean ± S.D. (error bars) from three independent biological repeats experiments, each of which included 4 technical repeats. #P < 0.05; ##P < 0.01; ###P < 0.001.