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. 2020 Jan 9;19:951–960. doi: 10.1016/j.omtn.2019.10.049

Figure 5.

Figure 5

Overexpression of miR-183-5p or Downregulation of FoxO1 in RAW264.7 Cells Reverses Exosome-mmu_circ_0001359-Induced Proinflammatory Cytokines and M2-like Macrophage Activation under LPS-Inducing Condition

(A and B) Quantitative real-time PCR shows the expression of miR-183-5p (A) and FoxO1 (B). Data are presented as the mean ± SEM. **p < 0.01, ***p < 0.001 versus the control group; #p < 0.05, ###p < 0.001 versus the LPS group; $$$p < 0.001 versus the LPS+circRNA-Exosome group. (C–F) The expression of inflammatory factors IL-1β (C), IL-6 (D), TNF-α (E), and MCP-1 (F) in RAW264.7 cell media was detected by ELISA. Results are expressed as mean ± SD (n = 4). **p < 0.01, ***p < 0.001 versus the control group; ##p < 0.01, ###p < 0.001 versus the LPS group; $$$p < 0.001 versus the LPS+circRNA-Exosome group. (G–L) qRT-PCR expression of M1 macrophage markers iNOS (G), TNF-α (H), and IFN-γ (I), and M2 macrophage markers Arg1 (J), Ym1 (K), and IL-10 (L). Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group; #p < 0.05, ##p < 0.01, ###p < 0.001 versus the LPS group; $p < 0.05, $$p < 0.01, $$$p < 0.001 versus the LPS+circRNA-Exosome group.