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. 2020 Jan 9;19:951–960. doi: 10.1016/j.omtn.2019.10.049

Figure 6.

Figure 6

Exosomes from mmu_circ_0001359-modified ADSCs attenuate airway remodeling by decreasing M1-like macrophage activation induced inflammatory cytokines and pulmonary fibrosis

(A) Collagen deposition and tissue fibrosis were assessed by Masson trichrome staining. (B) The relative fibrosis in different group were analysis. Data are presented as the mean ± SEM. ∗∗∗p < 0.001 versus control group. ##p < 0.01, ###p < 0.001 versus OVA group. $$$p < 0.001 versus the exosome treatment group. (C) Immunohistochemical staining of apoptosis. (D) The relative apoptosis in different group were analysis. Data are presented as the mean ± SEM. ∗∗∗p < 0.001 versus control group. #p < 0.05, ###p < 0.001 versus OVA group. $$$p < 0.001 versus exosome treatment group. (E) Immunofluorescence shows angiogenesis using CD31 staining. (F) The relative CD31 expression in different group were analysis. Data are presented as the mean ± SEM. ∗∗∗p < 0.001 versus Sham group. ###p < 0.001 versus OVA group. $$$p < 0.001 versus the exosome treatment group. (G–J) ELISA showing the expression of inflammatory markers IL-1β (G), IL-6 (H), TNF-α (I), and MCP-1 (J). Data are presented as the mean ± SEM. ∗p < 0.05, ∗∗∗p < 0.001 versus Sham group. #p < 0.05, ##p < 0.01, ###p < 0.001 versus OVA group. $$$p < 0.001 versus the exosome treatment group. (K–P) qRT-PCR expression of M1 macrophage markers [iNOS (K), TNF-α (L), and IFN-γ (M)] and M2 macrophage markers [Arg1 (N), Ym1 (O), and IL-10 (P)]. Data are presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 versus Sham group. #p < 0.05, ##p < 0.01, ###p < 0.001 versus OVA group. $$$p < 0.001 versus the exosome treatment group. (Q and R) RT-qPCR expression of miR-183-5p (Q) and FoxO1 (R). Data are presented as the mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001 versus Sham group. #p < 0.05, ###p < 0.001 versus OVA group. $$$p < 0.001 versus the exosome treatment group.