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. 2018 Nov 28;6(3):296–303. doi: 10.1016/j.gendis.2018.11.003

Figure 1.

Figure 1

ASL expression in HCC. (A and B) ASL protein and mRNA expression in five liver cancer cell lines, the immortalized liver cell line (MIHA), and PHH were measured by western blot and qRT-PCR, respectively. β-actin was used as a loading control for Western blot and as a reference gene for qRT-PCR. (C) Western blot analysis of ASL protein levels in 24 paired frozen HCC tissues (T) and adjacent nontumoral liver tissues (N). GAPDH was used as a loading control. (D) Quantitative analysis of ASL protein levels in 24 paired HCC tissues. **P < 0.01. (E) qRT-PCR analysis of ASL mRNA levels in 24 paired HCC tissues and adjacent nontumoral tissues. GAPDH was used as a reference gene. **P < 0.01.