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. 2018 Nov 28;6(3):296–303. doi: 10.1016/j.gendis.2018.11.003

Figure 2.

Figure 2

ASL knockdown inhibited HCC cell proliferation. (A) PLC/PRF/5 and Hep3B cells were infected with lentivirus expressing ASL-targeting shRNA (shASL) or control shRNA (shCont) for 3 days. ASL protein level was detected by western blot. β-actin was used as a loading control. (B and C) ASL knockdown significantly inhibited the proliferation of PLC/PRF/5 and Hep3B cells at indicated time points. Cell numbers were counted using a trypan blue exclusion assay. **P < 0.01. (D) Soft agar assay. PLC/PRF/5 and Hep3B cells were infected with lentiviruses as indicated and were grown for 3 weeks under antibiotic selection. The quantification of colonies was performed by counting visible colonies in each well and expressing then as a percentage relative to control cells. Columns show the mean values of triplicate experiments. **P < 0.01. (E) Colony-formation assay. PLC/PRF/5 and Hep3B cells infected with lentiviruses as indicated were cultured for 2 weeks and stained with crystal violet. All histograms show mean values from three independent experiments. **P < 0.01.