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. 2018 Nov 28;6(3):296–303. doi: 10.1016/j.gendis.2018.11.003

Figure 4.

Figure 4

ASL regulated Bax signaling in HCC cells. (A) PLC/PRF/5 cells were infected with lentiviruses as indicated for 3 days. mRNA microarray analysis indicated alternations in several key regulators in the Bax-mediated apoptotic pathway. β-actin mRNA expression was used as an internal control. *P < 0.05. **P < 0.01. (B) Western blot analysis of several components of the Bax-mediated apoptotic pathway in PLC/PRF/5 and Hep3B cells transduced with shASL or shCont. β-actin was used as a loading control. (C) PLC/PRF/5 cells were transfected with indicated siRNA for 3 days. The ASL and Bax protein levels were determined by western blot. β-actin was used as a loading control. (D) Gene silencing of Bax rescued the growth inhibition induced by ASL knockdown in PLC/PRF/5 cells. Cell numbers were counted using a trypan blue exclusion assay at indicated time points. *P < 0.05. **P < 0.01. (E) Bax knockdown abolished the induction of apoptosis in ASL-silenced PLC/PRF/5 cells. Apoptosis rate was analyzed by flow cytometry with Annexin V/PI. Columns show the mean values of triplicate experiments. **P < 0.01.