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. 2020 Jan 9;23(2):100827. doi: 10.1016/j.isci.2020.100827

Figure 4.

Figure 4

Interaction of Fragmented DNA with EPS Protein Results in Formation of Robust Biofilm

(A) SEM images of PAO1 biofilm at 12 h treated with 500 ng intact genomic DNA (iDNA) and fragmented genomic DNA (fDNA). Scale bar, 5 μm.

(B) Confocal microscopic images showing SYPRO Ruby (red) and TOTO-1 (green) staining of PAO1 biofilm at 12 h treated with 500 ng intact genomic DNA (iDNA) and fragmented genomic DNA (fDNA). The co-localization of EPS protein (red) and eDNA (green) is shown as white dots. Scale bar, 5 μm.

(C) Crystal violet assay of PAO1 biofilm at 12 h treated with 500 ng intact genomic DNA (iDNA), and fragmented genomic DNA (fDNA) (n = 8). Data are mean ± SD.

(D) SEM images of PAO1ΔwspF biofilm at 24 h treated with buffer and ATA. Scale bar, 5 μm.

(E) Confocal microscopic images showing SYPRO Ruby (red) and TOTO-1 (green) staining of PAO1ΔwspF biofilm at 24 h treated with buffer and ATA. The co-localization of EPS protein (red) and eDNA (green) are shown as white dots. Scale bar, 5 μm.

(F) Crystal violet assay of PAO1ΔwspF biofilm at 24 h treated with buffer and ATA (n = 8). Inhibition of DNA-protein interaction compromised in vitro PAO1ΔwspF biofilm formation. Data are mean ± SD.

See also Figures S5 and S6.