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Comparison of GV sPLA2 and cPLA2α mRNA in MΦWT and MΦTg. Peritoneal macrophages isolated from 8-week-old WT and Tg mice were treated with vehicle (Control) or IFNγ + LPS. The cells were harvested and processed for real-time qPCR analyses. A: sPLA2 GV (Control MΦWT 2−ΔΔCT, 1.01 ± 0.06). B: cPLA2α (Control MΦWT 2−ΔΔCT, 1.01 ± 0.07). Data are mean ± SEM of fold-change relative to control determined from four independent experiments. *MΦTg significantly different from MΦWT, P < 0.01.)
