Fig. 2.

Pro-inflammatory PG production by MΦ_WT and MΦ_KO. Peritoneal macrophages isolated from 8-week-old WT and KO mice were treated with vehicle [control (C)] or IFNγ + LPS and the media collected for lipidomics analyses. The data represent activated (A) fold-changes in lipids relative to C. A: Individual PGs. Control (pmol lipid/1e⁺⁰⁶) MΦ_WT and MΦ_KO: 6-keto PGF₁α, 2.512 ± 0.302 and 3.818 ± 0.564; TXB₂, 4.563 ± 0.585 and 4.491 ± 0.538; PGD₂, 0.568 ± 0.083 and 0.881 ± 0.128; 8-Iso PGF₂α, 0.056 ± 0.008 and 0.059 ± 0.013; 5-IPF₂α-VI, 0.263 ± 0.033 and 0.301 ± 0.068; PGE₂, 1.213 ± 0.313 and 1.690 ± 0.349; PGA₂, 0.472 ± 0.035 and 0.525 ± 0.050; 15-deoxyΔ12,14-PGJ₂, 0.253 ± 0.087 and 0.240 ± 0.072; PGF₂α, 0.646 ± 0.055 and 0.591 ± 0.032. B: PG pool. Control (pmol lipid/1e⁺⁰⁶) MΦ_WT and MΦ_KO: 3.804 ± 0.253 and 4.564 ± 0.443. Data are mean ± SEM determined from seven to nine independent experiments. *MΦ_KO significantly different from MΦ_WT, P < 0.05).