Fig. 4.

Production of anti-inflammatory PGE₁ and SPMs by MΦ_WT and MΦ_KO. Peritoneal macrophages isolated from 8-week-old WT and KO mice were treated with vehicle [control (C)] or IFNγ + LPS and the media collected for lipidomics analyses. The data represent activated (A) fold-changes in lipids relative to C. A: PGE₁ ± IFNγ + LPS. Control (pmol lipid/1e⁺⁰⁶) MΦ_WT and MΦ_KO: 0.162 ± 0.020 and 0.240 ± 0.021. B: SPMs. Control (pmol lipid/1e⁺⁰⁶) MΦ_WT and MΦ_KO: resolvin D2, 0.110 ± 0.029 and 0.100 ± 0.032; resolvin D1, 0.100 ± 0.013 and 0.115 ± 0.019; lipoxin A₄, 0.235 ± 0.065 and 0.288 ± 0.109. Data are mean ± SEM determined from seven to nine independent experiments. *MΦ_KO significantly different from MΦ_WT, P < 0.05.