Fig. 9.

Production of anti-inflammatory PGE₁ and SPMs MΦ_WT and MΦ_Tg. Peritoneal macrophages isolated from 8-week-old WT and Tg mice were treated with vehicle [control (C)], IFNγ + LPS, or IL-4 and the media collected for lipidomics analyses. The data represent activated (A) fold-changes in lipids relative to C. Data are mean ± SEM determined from four independent experiments. A: PGE₁ ± IFNγ + LPS. Control (pmol lipid/1e⁺⁰⁶) MΦ_WT and MΦ_Tg: 0.033 ± 0.011 and 0.017 ± 0.003. B: SPMs ± IL-4. Control (pmol lipid/1e⁺⁰⁶) MΦ_WT and MΦ_Tg: resolvin D2, 0.561 ± 0.073 and 1.074 ± 0.524; resolvin D1, 0.134 ± 0.042 and 0.136 ± 0.039; lipoxin A₄, 0.050 ± 0.008 and 0.050 ± 0.006. *MΦ_Tg significantly different from MΦ_WT, P < 0.05.