TABLE 1.
Name | Sequence (5′ to 3′) | Target | Product Size (bp) |
WT-PLA2G6_F | AGCTTCAGGATCTCATGCCCATC | WT (KO) iPLA2β | 1,400 |
WT-PLA2G6_R | CTCCGCTTCTCGTCCCTCATGGA | ||
KO-Neo_F | TCGCCTTCTATCGCCTTCTTGAC | KO-iPLA2β | 400 |
KO-Ex_R | GGGGCCTCAGACTGGGAATC | ||
WT-PLA2G6_F | CCTCCGGAGAGCAGCGATTAAAAGTGTCAG | WT (Tg) iPLA2β | 450 |
WT-PLA2G6_R | TAGAGCTTTGCCACATCACAGGTCATTCAG | ||
Tg-PLA2G6_F | CTAGGCTCAGACATCATGCTGGACGAGGT | RIP.iPLA2β.Tg | 200 |
Tg-PLA2G6_R | AAGATCTCAGTGGTATTTGTGAGCCAGGG |