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. 2019 Dec 5;61(2):229–243. doi: 10.1194/jlr.RA119000431

Fig. 5.

Fig. 5.

Relative expression of selected genes at the transcript level. Relative transcript levels in strain CBS.Δstp1 in relation to those in CBS 6938 (blue) and in strain CBS.cyp61−.Δstp1 in relation to those in CBS.cyp61− (light blue) (A) and in strain CBS.cyp61− compared with those in CBS 6938 (orange) (B). The transcript levels were evaluated by RT-qPCR after 120 h of culture in YM medium with constant agitation. The analyzed genes and GenBank numbers accession were HMGS [MK368600] and HMGR [MK368599] (MVA pathway), CYP51 [KP317478] (ergosterol biosynthesis), crtS [EU713462] (astaxanthin biosynthesis), crtR [EU884133] (ergosterol and astaxanthin biosynthesis), and SRE1 [MK368598] and STP1 [MN380032] (SREBP pathway). The genes grg2 [JN043364.1] and INV [FJ539193.2] (in an enclosed square), which are not likely regulated by Sre1N, were included as controls. Transcript levels were normalized to the housekeeping gene encoding β-actin [X89898.1] and expressed as fold-change compared with the levels in the corresponding parental strain using the 2−ΔΔCt method. Values are the average ± standard deviation of three independent cultures (*P < 0.01, **P < 0.05; Student’s t-test).