Fig. 4.

DiI-VLDL uptake is abrogated by treatment with heparin or Dynasore across BC cell lines. A: Treatment with heparin (500 µg/ml) or Dynasore (80 µM) reduced DiI-VLDL uptake, as quantified by flow cytometry. Replicates from ≥3 experiments are shown (***P < 0.001; one-way ANOVA with correction for multiple comparisons). B: Effects of heparin (500 µg/ml) and Dynasore (80 µM) on Di-VLDL binding and uptake in adherent BC cells visualized by confocal microscopy. Heparin or Dynasore prevented both binding and internalization of DiI-VLDL particles at 37°C. Representative images shown: DiI-VLDL (red), Hoechst nuclear stain (blue).