Fig. 3.

Effect of TUG891 and LA on ERK1/2 phosphorylation in cultured TBCs. Mouse (A) or human (B) TBCs were untreated (control) or treated with LA (50 μM) or TUG891 (10 μM) in combination and in the presence or absence of AH7614 followed by Western blot analysis for the determination of phosphorylated protein expression, as described in the Materials and Methods. The histograms show the results of independent experiments (n = 3). The difference between values was determined by one-way ANOVA, followed by LSD test. P < 0.05 was considered as statistically significant.