Fig. 5.

Assessment of peroxisomal biogenesis as well as oxidative and ER stress in human hepatocytes in which STK25 was depleted. IHHs were transfected with STK25 siRNA or NTC siRNA and challenged with oleic acid for 48 h. Representative images of cells processed for immunofluorescence with anti-PEX5 antibody (pink; A), anti-4-HNE antibody (green; B), or anti-KDEL antibody (pink, D), or stained with DHE (pink, C); nuclei stained with DAPI (blue). Scale bars, 40 μm (A–C) or 60 μm (D). Quantification of PEX5 (A), 4-HNE (B), DHE (C), and KDEL (D) staining. Results are the mean ± SEM from six wells per test condition. *P < 0.05, **P < 0.01, and ***P < 0.001 comparing cells transfected with STK25 siRNA versus NTC siRNA.