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. 2019 Nov 26;182(2):962–976. doi: 10.1104/pp.19.00688

Figure 5.

Figure 5.

Analysis of anther cutin and wax in the wild-type(WT) and ptc2-1 mutant. A, Total cutin and wax amounts per unit surface area in wild-type and ptc2-1 anthers. Error bars indicate ± sd (n = 5). B, Cutin monomers, amount per unit surface area in wild-type and ptc2-1 anthers. C, Wax constituents, amount per unit surface area in wild-type and ptc2-1 anthers. Error bars indicate ± sd (n = 5). **P < 0.01 by Student’s t-test. ALK, alkane; C16:0 FA, hexadecanoic acid; C18:0 FA, octadecanoic acid; C18:2 FA, linoleic acid; C18:3 FA, linolenic acid; C16:0 ω-HFA, 16-hydroxy-hexadecanoic acid; C18:1 ω-HFA, 18-hydroxy-octadecanoic acid; C16 DHFA, 16-dihydroxy-palmitic acid; C18:2 ω-HFA, 18-hydroxy-linoleic acid; C20:0 2HFA, 2‐hydroxyeicosanoic acid; cis‐9,10‐epoxy C18:1 ω-HFA, cis‐9,10‐epoxy‐18‐hydroxy‐oleic acid; cis‐9,10‐epoxy C18:0 ω-HFA,cis‐9,10‐epoxy 18‐hydroxy‐stearic acid; Chlorohydrin of 9,10-expoxy C18 ω-HFA, chlorohydrin of 9,10-epoxy-18-hydroxy-octadecanoic acid. Acids were analyzed as methyl esters, and hydroxyl groups were analyzed as trimethylsilyl esters.