Figure 3.

GI and ZTL temperature-dependent interactions. A and B, N. benthamiana plants were grown at 22°C under 16-h light/8-h dark cycles (LD) and protein extracts from leaves transiently expressing GI^Col-GFP and GI^Cvi –GFP (GI^Cvi) coexpressed with ZTL^Col or ZTL^Cvi were harvested at ZT12. Anti-GFP immunoprecipitates (IP) were probed with anti-ZTL polyclonal antibody (co-IP). Quantitation based on the ratio of co-IP/IP signals, normalized to strongest interaction. Image is representative of at least three trials; error bars represent ±se (n = 3). C and D, As in (A) and (B), but for plants maintained in 22°C or 27°C before extraction and processing. Statistical difference between pairs (Student’s t test) is shown as ^*P < 0.05, ^**P < 0.01. NS, not significant.