Figure 4.

Temperature-dependent ZTL accumulation depends on genotype. A to C, Seedlings (Col, Cvi) were grown for 7 d in 12-h light/12-h dark cycles (LD) at 22°C, continued at 22°C or 27°C for an additional 5 d under LD cycles, then sampled at the indicated ZT. Endogenous ZTL protein was probed with anti-ZTL polyclonal antibody. ZTL abundance was quantitated relative to a nonspecific band above the target protein and normalized to the most abundant level within each genotype for both temperatures. D, ZTL transcript abundance determined by reverse transcription quantitative PCR and normalized to ACTIN2. Cvi data are from four independent trials; two with Cvi-0 and two with Cvi-1 together with the corresponding Col accession. Statistical difference between pairs (Student’s t test) is shown as ^*P < 0.05, ^**P < 0.01. Error bars (B–D) represent ±se (n = 4).