Figure 3.

Protein composition of wild-type (WT) tobacco and transplastomic lines expressing β-cyanobacterial carboxysome components. A and B, Polypeptides in leaf extracts prepared from plants of each line were separated by denaturing SDS-PAGE (A) and nondenaturing native PAGE (B) and either stained with Coomassie Blue (top gels) or used for immunoblotting with antibodies against cyanobacterial Rubisco LSU (SeLSU) and CcmM35 and against the tobacco Rubisco small subunit (NtSSU; bottom gels). Images showing blotting of PAGE gels are slices from blots (Supplemental Fig. S5) and show the indicated size regions where the respective antibodies detect proteins of interest. For SDS-PAGE and native PAGE, 10 and 20 µg of total soluble protein was loaded per lane, respectively. Lanes marked M indicate protein markers containing proteins of a range of sizes as indicated at left of each gel. C, SDS-PAGE and native PAGE gels immunoblotted with antibody against NtSSU, loaded with 20 and 40 µg of total soluble protein, respectively.