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. 2019 Dec 16;182(2):1022–1038. doi: 10.1104/pp.19.01195

Figure 3.

Figure 3.

Constant chilling and diurnal freezing induce contrasting cold acclimation and freezing tolerance. A, Survival to freezing in Bd21-3, Bd30-1, Bd18-1, and Bd29-1 after 7 d in either control conditions (CTR; short-day 22°C), constant chilling (CC), or diurnal freezing (DF) measured in whole-plant freeze tests in which temperature hourly decreases by 1°C down to −12°C. B, Survival to freezing in Bd21-3 after 28 d in either control (CTR), constant chilling (CC), or diurnal freezing (DF). C, Relative transcript accumulation of C-REPEAT BINDING FACTOR 1 (CBF1), ICE-RECRISTALLIZATION INHIBITOR (IRI), and COLD-REGULATED410 (COR410) at 16 or 24 h after exposure to CC or DF. D, Nonstructural carbohydrate contents in CTR, CC-, and DF-treated plants after 7 d in either treatment. E, Pro contents in CTR, CC-, and DF-treated plants after 7 d in either treatment. Error bars represent sd among three biological replicates; different letters represent statistically significant differences; P < 0.05.