Figure 2.

The Ser-265Leu mutation in CT-β facilitates the formation of the CT–αβ subcomplex. SDS-PAGE analysis of extracts from E. coli strains coexpressing His-tagged CT-α with either wild-type CT-β or the CT-β(Ser-265Leu) mutant. The extracts were passed through a Ni-NTA affinity chromatography column, and the non-bound proteins were collected in the “Column Wash” fraction, while proteins that bound to the column were eluted with a His-containing buffer. Proteins were visualized by staining gels with Coomassie Brilliant Blue. The figure is a representative result of triplicate experiments that were sequentially conducted.