Figure 3.

Interdependence between BCCP1–BADC3–BC and CT–αβ subcomplexes to support the carboxylation of acetyl-CoA. A, Increasing concentration of purified CT–αβ subcomplex was titrated against a constant concentration of BC (0.62 μm) in the purified BCCP1–BADC3–BC subcomplex. B, Increasing concentration of purified BCCP1–BADC3–BC subcomplex was titrated against a constant concentration of purified CT–αβ subcomplex (2.18 μm). htACCase activity was measured as the rate of malonyl-CoA appearance using the MCR-coupled assay. Each data point represents the mean ± se (n = 3), and the experiment was duplicated with analogous results.