Effect of single and double badc mutants on fatty acid accumulation in mature seeds. Fatty acids were extracted from samples of 100 seeds. Each value is the mean of three to six biological replicates, and error bars indicate the mean ± se of the total fatty acid content. The sum of the minor fatty acid components (18:0, 20:0, 20:1[13-cis], 20:2, and 22:1) is indicated as “Others.” Student’s t test was used to determine the significance in the difference between the wild type (WT) and each indicated mutant (*P < 0.05; **P < 0.01). Asterisks labeled within the data bars represent the comparisons for individual fatty acid components, and asterisks labeled on top of the data bars represent the comparison for total fatty acid content.