Table 3. Hill analysis of the cooperativity between ATP and MgCl₂ in the carboxylation of acetyl-CoA catalyzed by htACCase.

ACCase activity was measured as the rate of malonyl-CoA appearance using the MCR-coupled assay. Data are the average ± se (n = 3).

Terms	[MgCl2] = [ATP]	[MgCl2] = [ATP] − 1.0 mm	[MgCl2] = [ATP] + 2.5 mm
Vmax	22.8 ± 0.3	22.5 ± 0.8	20.6 ± 0.5
n	1.9 ± 0.1	4.7 ± 0.7	2.8 ± 0.4
Khalf	0.45 ± 0.01	1.80 ± 0.07	0.26 ± 0.01