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Expression of Ma1G C-terminal truncation mutants in N. benthamiana leaves. A, Sequence alignment of tonoplast-localized ALMT members, including Ma1 and ma1. The arrows above the alignment indicate the residue positions of the various experimental truncation sites tested. B, The expression of Ma1G-GFP, ma1-GFP, and various Ma1G C-terminal truncation mutants with a C-terminal fused GFP in the A. tumefaciens-infiltrated N. benthamiana leaves. Bar = 100 µm.
