Figure 9.

Misregulation of MADS78 and MADS79 causes impairment of starch granules. A, Representative images of mature seeds from MADS78 and MADS79 mutants (left column; bar = 1 cm) and their cross sections (right column; bar = 20 µm) using SEM. Images shown were digitally extracted and scaled for comparison. B, RT-qPCR analysis of selected genes involved in carbon metabolism at 72 HAF in OE and knockout mutants relative to the wild type (WT). The genes were selected based on RNA-seq analysis. For statistical analysis, Student’s t test was used: ***, P < 0.001; **, P < 0.01; and *, P < 0.05. Error bars indicate sd (n = 6; two biological and three technical replicates).