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. 2019 Oct 28;182(2):1161–1181. doi: 10.1104/pp.19.00893

Figure 1.

Figure 1.

Analysis of protein interactions among PP2A-B′γ, CPK1, and SAG12. A, Schematic representation of full-length CPK1, a truncated VKm form that consists of the variable (V) N terminus and the kinase (K) domain, and the V N terminus that was found to interact with PP2A-B′γ in the yeast two-hybrid screen. The schemes and their domains are drawn to scale. B, BiFC analysis of protein interactions in N. benthamiana leaves transiently expressing the fusion protein pairs CPK1-YN/PP2A-B′γ-YC, CPK1-YN/PP2A-B′κ-YC, CPK1-YN/YFP-YC, CPK1-VKm-YN/PP2A-B′γ-YC, CPK1-VKm-YN/PP2A-B′κ-YC, and CPK1-VKm-YN/YFP-YC. C, BiFC analysis of protein interactions in Arabidopsis leaves. Transgenic Arabidopsis plants expressing the bacterial effector AvrPto under the control of a dexamethasone-inducible promoter (Hauck et al., 2003) transiently expressing the fusion protein pairs CPK1-YN/PP2A-B′γ-YC, CPK1-YN/PP2A-B′κ-YC, CPK1-VKm-YN/PP2A-B′γ-YC, and SAG12-YN/PP2A-B′γ-YC. Scale bars = 50 µm.