Figure 5.

Contents of SA and camalexin in apical and basal leaf halves of 6-week–old plants. Leaves of 6-week–old wild type, pp2a-b′γ, pp2a-b′γ P35S:PP2A-B′γ, pp2a-b′ζ, pp2a-b′γζ, pp2a-b′γ sid2, and sid2 were harvested and split into apical and basal halves. Metabolites were extracted in 85% methanol and the contents of SA (A) and camalexin (B) were analyzed by liquid chromatography tandem mass spectrometry. Data are means ± sd, n = 4. Statistically significant differences between apical or basal leaf halves of a mutant to the corresponding leaf halves of the wild type are indicated by asterisks. Statistically significant differences between the apical and the basal leaf halves of the same genotype are indicated by circles. ANOVA: leaf half, P < 0.001 (F = 26.48), genotype, P < 0.001 (F = 48.53), leaf half*genotype, P < 0.001 (F = 26.57); Tukey posthoc test: ^***P < 0.001, °°°P < 0.001.