TABLE 2.

Primers and standard used for amplification in one-step RT-qPCR to detect RV and TV bound to PMG-MBs

Step	Primer or standard	Sequence	Position in the genome	Fragment size (bp)
Quantitative PCR for RV OSU NSP3 for the binding assay	Forward primer JVKF	5′-CAGTGGTTGAT CT AAAT-3′	NSP3 (17–36)	130
	Reverse primer JVKR	5′-TCATTGTAATCATATTGAATACCCA-3′	NSP3 (123–147)
Standard curve for RV OSU NSP3	Standard sequence for RV OSU NSP3	5′-GGCTTTTAATGCTTTTCAGTGGTTGATGCTCAAGATGGAGTCTACTCAACAGATGGCATCTTCTATTATTAACTCTTCTTTTGAAGCTGCAGTTGTCGCTGCAACTTCTACATTAGAATTAATGGGTATTCAATATGATTATAATGAAGTATACACTAGAGTTAAAAGTAAGTTTGATTTTGTAATGGATGATTCTGGTG-3′	NSP3	1,305
Quantitative PCR for TV VP1 for the binding assay	Forward primer TVVP1-qPCR-F	5′-TTGCAGGAGGGTTTCAAGATG-3′	VP1 (5380–5400)	110
	Reverse primer TVVP1-qPCR-R	5′-CACGGTTTCATTGTCCCCCATA-3′	VP1 (5468–5490)